Bacteriophage T4 gene 44 DNA polymerase accessory protein. Sequences of gene 44 and its protein product.

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Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu.

Bacteriophage Mu baseplate protein gene product 44 (gp44) is an essential protein required for the assembly of viable phages. To investigate the roles of gp44 in baseplate assembly and infection, gp44 was crystallized at pH 6.0 in the presence of 20% 2-methyl-2,4-pentanediol. The crystals belong to space group R3, with unit-cell parameters a = b = 127.47, c = 63.97 A. The crystals diffract X-ra...

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DNA Synthesis on a Double-stranded DNA Template by the T4 Bacteriophage DNA Polymerase and the T4 Gene 32 DNA Unwinding Protein

T4 DNA polymerase cannot use a nicked duplex DNA molecule as a template-primer for DNA synthesis, apparently because it is unable to displace the 5’ end of the strand paired to the strand serving as the template. Addition of the T4 phage gene 32 DNA unwinding protein (32-protein) facilities strand displacement and allows the T4 DNA polymerase to synthesize DNA using nicked duplex T7 DNA as the ...

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DNA Synthesis on a Double-stranded DNA Template by the T4 Bacteriophage DNA Polymerase and the T4 Gene 32 DNA Unwinding Protein

T4 DNA polymerase cannot use a nicked duplex DNA molecule as a template-primer for DNA synthesis, apparently because it is unable to displace the 5’ end of the strand paired to the strand serving as the template. Addition of the T4 phage gene 32 DNA unwinding protein (32-protein) facilities strand displacement and allows the T4 DNA polymerase to synthesize DNA using nicked duplex T7 DNA as the ...

متن کامل

DNA Synthesis on a Double-stranded DNA Template by the T4 Bacteriophage DNA Polymerase and the T4 Gene 32 DNA Unwinding Protein

T4 DNA polymerase cannot use a nicked duplex DNA molecule as a template-primer for DNA synthesis, apparently because it is unable to displace the 5’ end of the strand paired to the strand serving as the template. Addition of the T4 phage gene 32 DNA unwinding protein (32-protein) facilities strand displacement and allows the T4 DNA polymerase to synthesize DNA using nicked duplex T7 DNA as the ...

متن کامل

DNA synthesis on a double-stranded DNA template by the T4 bacteriophage DNA polymerase and the T4 gene 32 DNA unwinding protein.

T4 DNA polymerase cannot use a nicked duplex DNA molecule as a template-primer for DNA synthesis, apparently because it is unable to displace the 5’ end of the strand paired to the strand serving as the template. Addition of the T4 phage gene 32 DNA unwinding protein (32-protein) facilities strand displacement and allows the T4 DNA polymerase to synthesize DNA using nicked duplex T7 DNA as the ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1984

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(17)42566-x